Expressions of PTENand MMP-7 、VEGF in Giant Cell Tumor of Bone ( GCT)

Objective 　To evaluate the expressions of PTEN ( phosphatase and tension homologue deleted on chromosome 10), MMP-7 (matrix metalloproteinases-7, MMP-7), VEGF (vascular endothelial growth factor) and their relationships with clinical factors, pathological types and metastasis, recurrence of giant cell tumor of bone ( GCT) . Methods 　The expressions of PTEN, MMP-7, VEGF in 65 cases of human GCT were detected by using immunohistochemical staining. The expressions of PTEN, MMP-7, VEGF and other clinical fractures were analyzed with the metastasis and recurrence of the tumor. Results 　The expressions of the PTEN, MMP-7, VEGF in GCT were 56. 95 % and 72. 30 %, 49. 20 %, respectively. In the clinical-pathological-X-ray groups, the expressions of PTEN were 83. 30 %, 68. 10 %, 28. 00 %, the positive rate had no significantly difference between the Non2malignant and doutful-malignant groups, P> 0. 05 ( P = 0. 271), but there were significantly difference between the doutful-malignant and malignant groups, P < 0. 05 ( P = 0. 001) ; the expression of MMP-7 were 39. 00 %, 72. 50 %, 96. 00 %, and the expression of VEGF were 16. 67 %, 54. 50 %, 68. 00 %, the positive rates of them had significantly difference, P < 0. 05 ( P = 0. 031, 0. 001) ; the expressions of PTEN, MMP-7, VEGF in the recurrence group were 33. 30 %, 93. 30 %, 86. 60 %, but in the nonrecurrence group were 64. 00 %, 66. 00 %, 38. 00 %, their expressions were statistically related to the recurrence of GCT, P < 0. 05 ( P = 0. 035, 0. 038, 0. 001) ; in the metastasis group, the expressions of PTEN, MMP-7, VEGF were 30. 00 %, 100 %, 85. 00 %, in the no metastasis group, the positive rates of PTEN, MMP-7, VEGF were 68. 80 %, 60. 00 %, 33. 30 %, the positive rates of them had significantly difference, P < 0. 05 ( P = 0. 003, 0. 001, 0. 001) ;at the same time, The expression of PTEN in GCT was inversely correlated with the expressions of VEGF and MMP-7, P <0. 05 ( P = 0. 001, 0. 02) ; However, the expressions of PTEN 、MMP-7 、VEGF had no statistically significance with the pathological types and sexuality. Conclusion 　The decreased expression of PTEN and increased expressions of MMP-7 、VEGF have significant relationship to the prognosis of GCT. The combined detection of PTEN and MMP-7 may be of important clinical value to evaluate the infilt rative ability and prognosis of GCT.