The Construction and Identification of siRNA Eukaryotic Expression Vector Targeting Genetic Imprinted Gene PEG10

Objective 　To construct and identify the siRNA eukaryotic expression vector targeting PEG10. Methods 　Four siRNAs were designed according to the coding sequence of PEG10 gene, and cloned into the downst ream of H1 promoter of psiRNA-hH1neo. The const ructed recombinant was analyzed and identified by Ase Ⅰendonuclease digestion and DNA sequencing. Results 　The constructed psiRNA plasmid digested with Ase Ⅰwas linearized. The sequencing result confirmed that the sequence of inserted fragment was correct . Conclusion 　Eukaryotic expression vector of siRNA targeting PEG10 gene was successfully constructed, and should be a novel effective expression vector for HCC gene therapy.