Immune Enhancement and Tumor Inhibition of S-180 Vaccine Combined with Different Microorganism Adjuvants

Objective To compare the immunoregulation effects and tumor inhibitions of Candida albicans(CA),Listeria monocytogenes(LM),and BCG which were used as adjuvents in the active specific immunotherapy of mice S-180 tumor. Methods S-180 tumor cells were treated with CA or LM and then prepared to vaccines(CA-S180 vaccine,LM-S180 vaccine).BCG-S180 vaccine was a mixture of formalin fixed tumor cell and BCG in proportion.Balb/c mice were inoculated with 1×105　S-180 tumor cells in footpads or in abdominal cavities followed by the immunotherapy of the described vaccines.IL-2,IFN-γ secreted by spleen cells and TNF-α secreted by peritoneal M of the immunized mice were determined.The weights of footpad tumors were recorded and the growth inhibitions were calculated.The average survival periods of tumor-bearing peritoneally mice were also reckoned. Results The levels of IL-2，IFN-γ,and TNF-α in group CA-S180，LM-S180,and BCG-S180 were much higher than that in control group S-180 and saline (P<0.01，0.05).The tumor growth in three groups was also inhibited with the range of 62.8%～75.8% inhibition ratios (P<0.01).The average survival period of mice in three groups enlonged to 7～14 days.CA-S180 vaccine and LM-S180 vaccine raised respectively the living ratios of tumor-bearing mice to 20% (2/8). Conclusion s These results indicated that Candida albicans,Listeria monocytogenes play a role in similar immunoenhance effects as BCG and inhibit effectively the growth of experimently transplanted tumor cooperating with S-180 vaccine