Sequence Analysis of the TCRβ CDR3 Region of T Cell Clones in Colorectal Cancer

Objective To study the molecular characteristics of anti-tumor T cell clones by analyzing the sequence of TCRβ CDR3 region from tumor-infiltrating lymphocytes (TIL) and peripheral blood lymphocytes (PBL) of patients with colorectal cancer. Methods The 24 TCR(V gene from TIL and PBL in pre-operation and post-operation were amplified by the method of combined reverse transcriptase-polymerase chain reaction (RT-PCR) with denaturation polyacrylamide sequencing gel eletrophoresis and gene sequencing,Which is ca...