Influence of Low Frequency Ultrasound on Proliferation and Apoptosis of Human Ovarian COC1

Objective To investigate the effect of Low-Frequency Ultrasound on proliferation and apoptosis of human ovarian cell line COC1. Methods The growth inhibitory effect of Ultrasound Exposure( 0.24 MHz, 5.12 W /cm 2× 5 s ) on COC1 cell was observed by MTT colormetric assay;Cell apoptosis was analyzed using flow cytometry(FCM);Terminal deoxynucleotidyl transferase-mediated dUTP-digoxin nick end labeling(TUNEL);Phase contrast light microscope and Electron microscope were applied to detect the morphological c...