Analysis of T Cell Clonal Proliferation and the Prognosis in Patients with Tumors

Objective To study T cell immunology in patients with solid cancers, the molecular features of clonal expanded T cell in TIL and PBL were analyzed by assessment of TCRβ gene repertoire. Methods The rearrangement of V J genes in TCRβ chain was amplified by the method of RT PCR with denaturation polyacrylamide sequencing gel eletrophoresis. The PCR production was sequenced directly to determine the characteristics of the clonal expanded transcripts. Results There were oligoclonal expanded transcripts of TC...