The study of specificity and stability of an anti-CD71 mouse/human chimeric antibody(Dd2C)-secreted transfectomas keeping in liquid nitrigen

Objective To study the specificity and stability of an anti CD71 mouse/human chimeric antibody (D2C) secreted transfectomas keeping in liquid nitrogen for tow years. Methods Analysis of the chimeric Ab production in culture supernatant was made by the standard concentration curve method with ELISA. The antibody (D2C) was purified by DEAE Sephredax A50 ion exchange chromatography from ascites and was confirmed by SDS PAGE . Results The production of the chimeric Ab(D2C) in the culture supernatant wa...