Study of the Cell Apoptosis and Mechanism of Plant Polysaccharide in Cellular Signalling Pathway

Objective 　To study the cell apoptosis and probe its under lying mechanism of plant polysaccharide in cellular signalling pathway. Methods 　After cultured for 1 and 8h, the cell apoptotic rate of mice sarcoma S-₁₈₀ ell line was observed by flow cytomet ry. Results 　cell apoptosis of mice sarcoma S- ₁₈₀ cell line was induced by PB (polyporusum bellatus) PPS (pachyman polysaccharides) APS (ast ragalus polysaccharides) in dose-and time-dependent manners. Af ter respectably treated with 100, 250, 500μg/ mL of Plant polysaccharide for 1 and 8h. The cell apoptotic rate of mice sarcoma S- ₁₈₀ cell line were on the rise with time and dose. The apoptotic rate of experiment groups have significant difference compared with control ( P < 0. 05) . Conclusion 　Plant polysaccharide may induce cell apoptosis of S- ₁₈₀ cell line in early phase and induce necrocytosis in late phase, inbihits proliferation of mice sarcoma S- ₁₈₀ cell.