Concentrations of intracellular second masseger Ca~(2+)i in human hepatocellular carcinoma cells and the effect on gap junctional intercellular communication

Objective To investigate the intracellular second masseger Ca2+i in human hepatocellular carcinoma cell lines HHCC,SMMC-7721 and normal liver cell line QZG. Methods Concentrations of intra- cellular second masseger Ca2+i in cell lines were examined in laser scanning confoeal microscopy after loaded by Fluo-3 AM. Scrape loading and dye transfer showed that QZG cells hepatoma cell lines Lucifer Yellow scrape loading and dye transfer were used to detect function of gap j...