Celecoxib inhibits proliferation and induces apoptosis via cyclooxygenase-2 and PGE_2 pathway in human pancreatic carcinoma cell line JF-305

Objective To evaluate the effects and mechanisms of celecoxib in inducing proliferation inhibition and apoptosis on human pancreatic carcinoma cells. Methods The anti-proliferative effect was measured by using Methabenzthiazuron (MTT) assay. Cell cycle and apoptosis were analyzed by using flow cytometry. The prostaglandin E 2 (PGE 2) levels in the supernatant of cultured pancreatic carcinoma cells were quantitated by enzyme-linked immunoabsordent assay (ELISA). Results Celecoxib suppressed the productio...