Inhibitory Effect of Antiprogestins Mifepristone on the Proliferation of Endometrial Carcinoma HHUA Cell Line in Vitro

Objective 　To investigate the effects and its mechanisms of antiprogestins mifepristone on the proliferation of human endomet rial carcinoma cell in vitro. Methods 　Human endometrial carcinoma HHUA cell were cultured in vitro and t reated with mifepristone in different concent ration for 24～96 hours. Methyl thiozolyl tet razolium (MTT) was used to observe the growth suppressive rate of HHUA cell. The expression of Ki-67 and c-myc of HHUA cell were determinded by immunohistochemist ry. Results 　The proliferation of HHUA cell were suppressed with mifepristone in time2dose dependent fashion in vitro ( P < 0. 05) . The decreased expression of Ki-67 and c-myc appeared when the mifepristone ≥5 μmol/ L treated the cell for 24 hours ( P < 0. 05) . Conclusion 　Antiprogestins mifepristone could significantly suppress the proliferative activity of human endomet rial carcinoma HHUA cell in time-dose dependent fashion in vitro. The action mechanisms of mifepristone were closely associated with the decrease of the expression of Ki-67 and c-myc.