Identification and Sequence Analysis of Fused scFv Antibody Binding Specifically to HSP70

Objective To identify the specific fused antibodies binding to HSP70 extracted from lung cancer by biopanning from a human scFv phage library of lung cancer. Methods HSP70 extracted from lung cancer was used as antigen to biopan human scFv phage library of lung cancer after four rounds of biopanning.High affinity and specificity positive monoclonical phage antibody was identified by ELISA.PCR was carried out for identification of the positive clones which combined with scFv gene sequence.DNA sequencing of po...