Cytotoxicity Induced by Dendritic Cells Pulsed with Acid Eluted Tumor Antigen Peptides against Bladder Cancer Cell Line

Objective To observe specific cytotoxicity induced by dendritic cells pulsed with acid eluted tumor antigen peptides against bladder cancer cell line. Methods The antigen peptides of BIU-87 cells were obtained by mild acid eluted technique.DCs were cultured with GM-CSF and IL-4 and TNF-α from healthy human peripheral blood monouclear cells in vitro,then pulsed with bladder-tumor peptides.Tumor specific CTLs were stimulated by DCs pulsed with bladder-tumor peptides and cytotoxicity caused by the CTLs against ...