Inhibition Effects of siRNA on Expression of Somatostatin Gene

Objective To study the somatostatin (SOM) gene expression inhibition of siRNA. Methods According to the gene sequene of SOM in the Genbank,we designed the targeted siRNA templates. The siRNA was synthesized by T7RiboMAX~ TM Expression RNAi system in vitro and was transfected into gastric cancer cell line BGC-823.The inhibitory effects were detected by RT-PCR and Immunohistochemical techniques. Results SiRNA inhibited SOM gene expression. Conclusion SiRNA was generated in vitro and it inhibited SOM gene expre...