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ZHENG Hong, LI Ming-shan, ZHAO Guo-qiang, DONG Zi-ming. Study On DNA Polymeraseβ Gene Mutation in Human Nasopharyngeal Cancer[J]. Cancer Research on Prevention and Treatment, 2007, 34(05): 340-341. DOI: 10.3971/j.issn.1000-8578.2820
Citation: ZHENG Hong, LI Ming-shan, ZHAO Guo-qiang, DONG Zi-ming. Study On DNA Polymeraseβ Gene Mutation in Human Nasopharyngeal Cancer[J]. Cancer Research on Prevention and Treatment, 2007, 34(05): 340-341. DOI: 10.3971/j.issn.1000-8578.2820

Study On DNA Polymeraseβ Gene Mutation in Human Nasopharyngeal Cancer

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  • Corresponding author:

    LI Ming-shan

  • Received Date: May 09, 2006
  • Revised Date: November 09, 2006
  • Objective  To study DNA polymeraseβ(polβ) gene mutation in human nasopharyngeal cancer. Methods  40 cases of nasopharyngeal carcinoma (NPC) tissues were collected to study DNA polβgene mutation with PT-PCR, PCR-SSCP and DNA sequence analyzing technique, the 40 cases of NPC were divided into three groups according to pathology differentiation, 16 cases of grade Ⅰ( G1), 14 cases of grade Ⅱ( G2), 10 cases of grade Ⅲ( G3) . Results  Polβgene mutation was detected by SSCP in 13 of 40 NPC tissues (with mutation rate 32. 5 %) and Many kinds of mutation were found by DNA sequencing, the mutation rate of G1, G2, G3 was 12. 5 %, 21. 4 %, 80 % respectively. The sequencing of the PCR product showed an T to C chang at nucleotide 454, which resulted in a substitution of the amino acid from Phe to Ser at site 114 ; 466nt G→A resulting in Gly →Glu at 118 site ;148nt A →G resulting in Asn→Ser at 28 site. Conclusion  The study proves there is polβalternation in human nasopharyngeal carcinoma, this induces change in amino acid and spatial st ructure. The mutation rate in low differentiated cancer was significantly higher than those in moderate and high ones. These mutations may be related with the development of NPC.
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