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WU Jun, WANG Xiao huai, YANG Tai cheng, LAI Huang wen, YANG Chuan hong, WANG Jie, ZHEN Wen ling. Calcium Ionophore- treated Human Peripheral Blood Mononcytes Rapidly Acquire Activated Dendritic Cells[J]. Cancer Research on Prevention and Treatment, 2004, 31(07): 384-386. DOI: 10.3971/j.issn.1000-8578.280
Citation: WU Jun, WANG Xiao huai, YANG Tai cheng, LAI Huang wen, YANG Chuan hong, WANG Jie, ZHEN Wen ling. Calcium Ionophore- treated Human Peripheral Blood Mononcytes Rapidly Acquire Activated Dendritic Cells[J]. Cancer Research on Prevention and Treatment, 2004, 31(07): 384-386. DOI: 10.3971/j.issn.1000-8578.280
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Calcium Ionophore- treated Human Peripheral Blood Mononcytes Rapidly Acquire Activated Dendritic Cells

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  • Objective To explore the effect of A 23187 (calcium ionophore, CI) on dendritic cells (DC) from healthy human peripheral blood monocytes. Methods Peripheral blood monocytes from healthy donors were treated with rhGM CSF of 100 ng/ml、rhGM CSF of 100ng/ml plus IL 4 of 50ng/ml and rhGM CSF plus A 23187 100ng/ml each, respectively. After cumtivation of 40 hours, change of cellular morphology was observed under light microscopy and electron microscopy. Surface markers on DCs were analyzed by flow...
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