Change of Fas Expression and Intracellular Calcium Content in Human Cervical Carcinoma Cells Treated with Arsenic Trioxide

Objective 　The study was to research Fas expression and int racellular calcium content change in human cervical carcinoma Hela cells t reated with arsenic t rioxide. Methods 　MTT method was used to observe the growth inhibition. Apoptosis was detected with Annexin V-FITC + PI dual parameter. Fas positive rate and int racellular calcium ions ( IECa ²⁺ ) levels were measured by flow cytometry in Hela cells treated with As₂O₃ . Results 　As₂O₃ could inhibit the growth of Hela cells dramatically. There was obvious dosage-effect and time-effect correlations, it s half inhibitory concent ration ( IC50 ) at 24h, 48h and 72h were 8. 62μmol/ L, 6. 77μmol/ L and 4. 89μmol/ L respectively. Apoptosis rate increased in Hela cells t reated with As₂O₃ . As₂O₃ could increase Fas expression and IECa ²⁺ levels in Hela cells ( P < 0. 01) . Conclusion 　As₂O₃ could inhibit the growth of Hela cells dramatically and induce apoptosis, we proposed that its mechanisms were probably associated with the improved Fas expression and IECa ²⁺ levels.