The Construction and Expression of Eukaryotic Expression Plasmid for Human RASSF2

Objective To construct eukaryotic expression plasmid of human Ras association domain family 2 gene for further study on RASSF2 function on tumor cell apoptosis and cell cycle arrest. Methods The RASSF2 gene fragment was amplified by RT PCR (from normal human peripheral blood )and cloned into the eukaryotic expressing vector pcDNA 3.1 /His C by DNA recombination techniques, and then PANC 1 cells were transfected with reconstructive plasmid by liposome mediated gene transfer method. RT PCR was used to ...