Effects of Recombinant Human Bone Morphogenetic Protein-2(rhBMP-2) on Apoptosis of Glioma Cell SHG-44 in Vitro

Objective To investigate the effects of rhBMP-2 on apoptosis of SHG-44 glioma cell. Methods We incubated cultured human SHG-44 glioma cell with.rhBMP-2 protein.The ultrastructural changes of the SHG-44 cell were examined by electron microscopy.The cell cycle was detected by flow cytometry and changes of DNA segment were analyzed by gel electrophoresis. Results The ultrastructural changes showed compaction of nuclear chromation.An apoptosis peak in sub G1 phage was observed.The agarose gel electrophoresis of cellular DNA showed a characteristic “ladder”. Conclusion rhBMP-2 can induce apoptosis of human SHG-44 glioma cell in vitro.