The effect of ~(⁶⁰)Co irradiation on the proliferation of and TNF-α secreting in TNF-α gene-transduced human liver cancer cells

Objective To make a tumor vaccine using _⁶⁰ Co irradiated human tumor necrosis factor alpha (TNF α) gene transduced human liver cancer cells(BEL 7404 TNF cells). Methods 60 Co were applied in different ways and different dosages to irradiate BEL 7404 TNF cells and the parented BEL 7404 cells, and the growth and the secreting amount of TNF α of tumor cells were observed after irradiating. Results The BEL 7404 TNF cells were alive and preserved the ability to secrete TNF α f...