In Vivo Experiment on Brain Glioma Cells Transfected with Green Fluorescent Protein

Objective To determine whether fluorescence from C6 glioma cells transfected with the enhanced green fluorescent protein gene in vitro and xenotransplanted into the brain of SD rats would permit the detection of brain tumor invasion in vivo. Methods C6 glioma cells were transfected with a plasmid vector (pEGFP-N3) containing the EGFP gene. Stable EGFP-expressing clone were isolated and stereotactically injected into the parenchyma of rats. Four weeks later,rats were killed and continuous brain sections ...