Anticancer Drug Sensitivity Test of Malignant Plcural and Ascitic Fluid with MTT Colorimetric Method

Chemosensitivity of 39 samples of malignant ascitic fluid to 10 anticancer drugs were assayed in vitro using MTT colorimctric method.Malignant plural fluid or ascitic fluid was collected in sterilized bottles containing 10 units of haparin for each milliliter of malignant fluid.Density gradient centrifugation was used to separate malignant cell from blood stained effusions.Single-cell suspensions were prepared and tumor cell inhibition rates were determined by MTT assay.It was shown that there was no statistically significant difference in anticancer drug sensitivity between the solid tumor and malignant fluid affected anticancer drugs sensitivity and must be seperated.By intraplcural of intraascitic injection of sensitive drugs,80% of these cancer patients obtained a short-term improvement.