The Affection of survivin and bcl-2 mRNA Expression on Drug- resistance of Gastric Cancer Cell Line MGC803 to Cisplatin

Objective 　To investigate the drug-resistance mechanisms of gastric cancer cell to cisplatin (CDDP) . Methods 　Gast ric cancer cell line MGC803 were t reated by CDDP (0. 1, 1. 0, 10. 0mg/ L) for 12, 24, 48 and 72hours. Proliferation was detected by MTT assay and apoptosis was measured by fluorescence staining. Cell cycle was detected by flow cytomet ry. survivin and bcl-2 mRNA expression were detected by RT-PCR. Results 　High concent ration of CDDP (10. 0mg/ L) could inhibit proliferation and induce apoptosis, but low concent ration of CDDP(0. 1, 1. 0mg/ L) did not . the effect s of inhibiting proliferation and inducing apoptosis did not increase significantly af ter 24 hours, The cell cycle was altered af ter the cells were t reated by CDDP. S phase of cells were increased and G2 / M phase of cells were decreased. survivin mRNA expression increased and bcl22 mRNA expression decreased gradually af ter t reated by CDDP(1. 0mg/ L) for 24h ( P < 0. 05) . Conclusion 　CDDP could arrest the cell cycle on MGC803 cell. But the cells were easily developing drug-resistance to low concent ration of CDDP. Over expression of survivin mRNA may be one of the mechanisms of drug resistance in MGC803 cells.