A study on anti-hepatocarcinoma effect Induced by vaccines made up of cells treated by method of TNF-α gene modifying combined with freezing

Objective To investigate the possibility of producing vaccine with higher effect of anti-hepatocarcinoma by means of freezing the tumour cells which have been TNF-α gene modified before. Methods The A and B vaccine were H22 cells modified by TNF-α genes and were treated by means of freezing, or not. The C and D vaccine were the H22 cells were modified by TNF-αgenes or were treated by means of freezing or not. Effects of these four kinds of vaccines on anti- plant-hepatocarcinoma in mice were compa...