Experience of technique on terminal transferase deoxyuridine nick-end

Objective To investigate the optimal conditions on apoptotic cells detected by terminal transferase deoxyuridine nick-end labeling (TUNEL). Methods 20 human large intestinal carcinomas and 20 rat cerebrum tissues were seprated into two groups. Group A was constituted of 10 human large intestinal carcinomas and 10 rat cerebrum tissues, the rest served as group B. Group A was treated according to conditions recommended by Boehringer Mannheim company, group B was treated according to our laboratory improv...