The Complexity of the Abnormal Tyrosine Kinase Signaling Pathway in Lung Adenocarcinoma

Objective To study the abnormity of tyrosine kinase signaling pathway in lung adenocarcinoma. Methods Total RNA was extracted form 10 lung adenocarcinoma samples and matched normal tissues. cDNA was labeled fluorescence during reverse transcription and hybridized on 20 slides of microarray with 13824 genes. After washed and scanned, the cy3∶cy5 ratios were obtained by computer analysis. Each experiment was repeated two times and the mean value was chosen. At last we analysis the expression of many compone...