Anti-hepatocarcinoma Therapeutical Effect of Tumor Special CTL Activated by Two Kind of Dendritic Cells in Nude Mice
-
Graphical Abstract
-
Abstract
Objective To study diference of cytomorphological,cellular ultrastructure and the immune response in vivo and vitro induced by diverse dendritic cells(DC) pulsed with tumor extracts. Methods Isolated and purified two kind of DC from peripheral blood by combination of granulocyte /macrophage colony stimulating factor,interleukin 4 and tumor necrosis factor α;extracted tumor-associated antigen from human hepatoma cell line SMMC-7721 cells;initiated the T lymphocyte with DC pulsed by the soluble tumor antigen t...
-
-