Inhibition of the Expression of survivin in Osteosarcoma Cell by A Short Hairpin RNA

Objective To construct a expression plasmid of a short hairpin RNA targeting survivin,and investigate its inhibitory effect on the expression of survivin in osteosarcoma cell line MG-63 in vitro. Methods Construct a expression plasmid of a short hairpin RNA targeting survivin,and transfect it into MG-63 cell by dosper liposomal.The expression of survivin mRNA and protein were examined by RT-PCR and immunohistochemical method,respectively.The inhibition of the cell proliferation was estimated by MTT method.Re...