Primary Study of Mouse Zinc Finger Gene zfp637

Objective To investigate the impact of zfp637 on proliferation of cancer cells. Methods The expression of zfp637 in normal tissues and cancer cell lines was examined by semi-quantity RT-PCR analysis.Small double strands interfering RNA(siRNA) was synthesized,and then screen the best interfering site.After that,proliferation of RNAi treated EMT6 cells was analyzed via 96-well plate approach.The assay was performed on day 1~4. Results zfp637 was expressed moderately in most normal tissues,highly expressed in Yac-1, EMT6, B16, H22, LL/ 2 and Hepal-6, but not expressed in pe-ripheral blood leukocyte. The z f p637-specific siRNAs substantially reduced the proliferation of EMT6 cells on the 4th day. Conclusion 　z f p637 was expressed at different levels in multiple tissues and various cancer cell lines. It probably functions as a positive regulator of cellular proliferation. Down regulation of it s expression in EMT6 cancer cells by RNAi led to a remarkable reduction of proliferation.