Effect of Gensenoside Rg3 on Apoptosis and Expression of HIF-1α and VEGF in Hep2 Human Laryngeal Cancer Cell Line under Anoxic Conditions

Objective 　To study the mechanism and effect of gensenoside Rg3 on Hep2 Cell Line during the normoxia and hypoxia. Methods 　Anoxic cultured Hep2 human laryngeal cancer cell line, mean-while the normal cont rol group and positive cont rol group (DDP) were set . The cell apoptotic was detected by TUNEL method. The cell cycle and cell apoptosis analysis was detected by FCM. Then the expression of HIF-1αand VEGF protein was detected by immunohistochemistry and flow cytomet ry ; the expression of HIF-1αand VEGF mRNA was detected by t ranscription-polymerase chain reaction (RT-PCR) . Results 　The apoptosis rate obviously increase by TUNEL and FCM( P < 0. 05) . Compared with the Hep2 cells with-out any induction, the expression of HIF21αand VEGF protein in Rg3 group s and DDP groups obviously depressed ( P < 0. 05) ;the expression of HIF21αand VEGF mRNA in Rg3 group s obviously depressed ( P < 0. 05) ;but had no effect on the level of HIF21α and VEGF mRNA in DDP groups. Conclusion Rg3 could inhibit the high protein and mRNA expression of HIF-1αand VEGF and it could induce cell apopto-sis under anoxic conditions, which may be one of it s anticancer mechanisms.