Study of IFN-β Gene Inducing Nude Mice of Human SHG44 Glioma Up-regulating of Fas and Cell Apoptosis

Objective 　To study the effect s of interferon-β( IFN-β) gene inducing human gliomas apoptosis and to observe whether interferon-βcan induce up-regulating of Fas expression. The relationship between cell apoptosis inducing by IFN-β gene and up-regulating of Fas expression was discussed. Methods 　To establish the SHG44 glioma model of nude mice. Eukaryonic expression vector (pSV2 IFNβ) for IFN-β packaged by liposome was injected into SHG44 glioma of nude mice ;and observe the glioma growth and calculate tumor size. The effects of IFN2β gene inducing human gliomas apoptosis and Fas expression were realized by immunohistochemistry and tunel. Results 　The expression of IFN-β gene in the glioma nude mice were determined. The glioma growth of nude mice was inhibited. IFN-βcan induce glioma cell SHG44 apoptosis. Fas expression of glioma cell is significantly higher than that of the cont rol group. Conclusion 　IFN-βgene can inhibit the growth of human gliomas and induce the apoptosis of human glioma cells. The apoptosis inducing by IFN-β gene may depend on the up-regulating of Fas expression.