Derivative of recombinant human tumor necrosis factor-α therapy for rat glioma

Objective To investigate the effects of derivative of recombinant human tumor necrosis factor-α(rhTNF-α) therapy for rat glioma. Methods rhTNF-α were created by modifying TNF-α, were cultured in culture medium conclude rhTNF-α in different concertration. The livability of cells was determined by MTT technique five days later and implanted C 6 cells in rat s brain to establish glioma model. rhTNF-α were intraperitoneally injected 10 days after implanted. The volume of glioma were measured by MRI 10 days a...