Effects of Sodium Cantharidinate on Proliferation and Apoptosis of Gastric Cancer Cells by Inhibiting JAK2/STAT3 Pathway

LIU Xinghong; LIU Jin; CHEN Haiyan; GUO Yuhang

doi:10.3971/j.issn.1000-8578.2024.24.0294

Cancer Research on Prevention and Treatment > 2024 > 51(11): 913-917. > DOI: 10.3971/j.issn.1000-8578.2024.24.0294

LIU Xinghong, LIU Jin, CHEN Haiyan, GUO Yuhang. Effects of Sodium Cantharidinate on Proliferation and Apoptosis of Gastric Cancer Cells by Inhibiting JAK2/STAT3 Pathway[J]. Cancer Research on Prevention and Treatment, 2024, 51(11): 913-917. DOI: 10.3971/j.issn.1000-8578.2024.24.0294

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Effects of Sodium Cantharidinate on Proliferation and Apoptosis of Gastric Cancer Cells by Inhibiting JAK2/STAT3 Pathway

1.
Department of Ultrasound Intervention, Xi'an International Medical Center Hospital, Xi'an 710001, China
2.
Department of Oncology, Xi'an International Medical Center Hospital, Xi'an 710001, China

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Received Date: April 02, 2024
Revised Date: May 18, 2024
Accepted Date: August 11, 2024

Graphical Abstract

Abstract

Abstract

Objective
To study the effects of sodium cantharidinate (SC) on the proliferation and apoptosis of gastric cancer cells through JAK2/ STAT3 pathway.
Methods
Gastric cancer cell line SGC-7901 was cultured and treated with different concentrations of SC (0.25, 0.5, 1.0, 2.0, 4.0, 8.0, and 16.0 μmol/L) and then transfected with control plasmid or JAK2 plasmid. Cell survival rate, apoptosis rate, and the expression levels of p-JAK2, p-STAT3, p-p38, p-ERK, and p-JNK were detected after 48 h of treatment.
Results
The results indicated that 1.0, 2.0, 4.0, 8.0, and 16.0 μmol/L of SC inhibited cell proliferation, and the survival rate decreased with an increase in SC concentration (P<0.05). SC doses of 1.0, 2.0, and 4.0 μmol/L were selected for the subsequent experiments. Compared with the control group, the apoptosis rate of the 1.0 μmol/L SC group exhibited no significant difference (P>0.05), while those of the 2.0 and 4.0 μmol/L SC groups increased significantly (P<0.05). The expression levels of p-JAK2 and p-STAT3 significantly decreased (P<0.05), while no significant difference was noted in the expression levels of p-p38, p-ERK, and p-JNK (P>0.05) in the 1.0, 2.0, and 4.0 μmol/L SC groups. The JAK2 plasmid was transfected simultaneously with the 4.0 μmol/L SC treatment; the expression levels of p-JAK2 and p-STAT3 and the survival rate increased, whereas the apoptosis rate decreased (P<0.05).
Conclusion
SC inhibits the growth and promotes the apoptosis of gastric cancer cells, and its mechanism may be related to the inhibition of JAK2/STAT3 pathway activation.
- Gastric cancer,
- Sodium cantharidininate,
- Proliferation,
- Apoptosis,
- JAK2/STAT3 pathway

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Competing interests: The authors declare that they have no competing interests.

References (16)

References

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Effects of Sodium Cantharidinate on Proliferation and Apoptosis of Gastric Cancer Cells by Inhibiting JAK2/STAT3 Pathway