Expression of Circular RNA hsa_circ_0018574 in Colorectal Cancer Tissues and Its Effect on Proliferation of Colorectal Cancer Cells

MA Rui; TIAN Jinhai; MA Rong; WAN Qiaofeng; LIU Hetao; WANG Libin

doi:10.3971/j.issn.1000-8578.2022.22.0516

Cancer Research on Prevention and Treatment > 2022 > 49(12): 1258-1264. > DOI: 10.3971/j.issn.1000-8578.2022.22.0516

MA Rui, TIAN Jinhai, MA Rong, WAN Qiaofeng, LIU Hetao, WANG Libin. Expression of Circular RNA hsa_circ_0018574 in Colorectal Cancer Tissues and Its Effect on Proliferation of Colorectal Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2022, 49(12): 1258-1264. DOI: 10.3971/j.issn.1000-8578.2022.22.0516

Citation:

PDF (5100 KB)

Expression of Circular RNA hsa_circ_0018574 in Colorectal Cancer Tissues and Its Effect on Proliferation of Colorectal Cancer Cells

1.
School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China
2.
Biochip Engineering Research Center, General Hospital of Ningxia Medical University, Yinchuan 750004, China

Funding:

Science Research Project of Ningxia Higher Education NGY2018081

National Natural Science Foundation of China 81860470

Ningxia High-level Science and Technology Innovation Leading Talent Project KJT2019003

More Information

Corresponding author:
WANG Libin, E-mail: wanglibin007@126.com
Received Date: May 12, 2022
Revised Date: September 05, 2022
Available Online: January 12, 2024

Graphical Abstract

Abstract

Abstract

Objective
To investigate the expression of hsa_circ_0018574 in colorectal cancer tissues and human colon cancer HT29 cell line, as well as its effect on the proliferation and apoptosis of colorectal cancer cells.
Methods
The circPrimer 1.2 software was used to draw the circRNA sequence structure. Meanwhile, the circRNA microarray was used to screen differentially-expressed circRNA in colorectal cancer tissues and adjacent tissues, and RNA was extracted from tissue samples. The expression of hsa_circ_0018574 in human colorectal tumors was detected by RT-qPCR. The si-circ_0018574 was transfected into HT29 cells, and the expression of CDK2, CDK4, CDK6, cyclinD1, and cyclinE cyclins were detected by colony formation assay, flow cytometry, and Western blot, respectively.
Results
The expression of hsa_circ_0018574 in human colorectal tumor tissues was significantly higher than that in adjacent tissues (P < 0.01). Meanwhile, the si-circ_0018574 in HT29 cells could significantly inhibit the proliferation of cancer cells, reduce clone formation and colony formation ability (P < 0.01), and induce tumor cell apoptosis (P < 0.01). The expressions of CDK2, CDK4, CDK6, cyclinD1 and cyclinE cyclins were decreased.
Conclusion
The hsa_circ_0018574 is highly expressed in colorectal tumors, and si-circ_0018574 could significantly inhibit the proliferation of HT29 cells, reduce cell division, and induce apoptosis.
- CircRNA,
- Colorectal cancer,
- Cell cycle,
- Proliferation,
- Apoptosis

FullText(HTML)

Competing interests: The authors declare that they have no competing interests.

References (20)

References

[1]	Sung H, Terlay J, Siegel RL, et al. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries[J]. CA Cancer J Clin, 2021, 71(3): 209-249. doi: 10.3322/caac.21660
[2]	刘宗超, 李哲轩, 张阳, 等. 2020全球癌症统计报告解读[J]. 肿瘤综合治疗电子杂志, 2021, 7(2): 1-13. https://www.cnki.com.cn/Article/CJFDTOTAL-ZLZD202102001.htm Liu ZC, Li ZX, Zhang Y, et al. Interpretation on the report of the Global Cancer Statistics 2020[J]. Zhong Liu Zong He Zhi Liao Dian Zi Za Zhi, 2021, 7(2): 1-13. https://www.cnki.com.cn/Article/CJFDTOTAL-ZLZD202102001.htm
[3]	李轩, 荣宝海, 解广东, 等. 环状RNA与结直肠癌相关性研究进展[J]. 中国中西医结合外科杂志, 2021, 27(1): 154-158. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZX202101038.htm Li X, Rong BH, Xie GD, et al. Research progress on the relationship between circular RNA and colorectal cancer[J]. Zhongguo Zhong Xi Yi Jie He Wai Ke Za Zhi, 2021, 27(1): 154-158. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZX202101038.htm
[4]	王莹, 郭雄, 王民, 等. 原发性骨性关节炎膝关节软骨细胞差异表达circRNA, miRNA筛选和生物学功能及其相互作用分析[J]. 山东医药, 2019, 59(21): 1-6. https://www.cnki.com.cn/Article/CJFDTOTAL-SDYY201921001.htm Wang Y, Guo X, Wang M, et al. Screening of differentially expressed circRNA and miRNA in chondrocytes of knee joint in primary osteoarthritis and biological function and interaction analysis of them[J]. Shandong Yi Yao, 2019, 59(21): 1-6. https://www.cnki.com.cn/Article/CJFDTOTAL-SDYY201921001.htm
[5]	朱磊, 董伦, 张恒柱. 环状RNA在胶质瘤中的研究进展[J]. 转化医学电子杂志, 2018, 5(7): 39-43. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHDZ201807008.htm Zhu L, Dong L, Zhang HZ. Research progress of circular RNA in glioma[J]. Zhuan Hua Yi Xue Dian Zi Za Zhi, 2018, 5(7): 39-43. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHDZ201807008.htm
[6]	夏立军, 衣服新, 翟旭, 等. 环状RNA同源域相互作用蛋白激酶3(circHIPK3)通过吸附miR-124-3p促进神经胶质瘤细胞增殖及转移[J]. 细胞与分子免疫学杂志, 2020, 36(7): 609-615. Xia LJ, Yi FX, Zhai Xu, et al. Circular RNA homeodomain-interacting protein kinase 3(circHIPK3) promotes growth and metastasis of glioma cells by sponging miR-124-3p[J]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2020, 36(7): 609-615.
[7]	Zhang C, Zhou X, Geng X, et al. Circular RNA hsa_circ_0006401 promotes proliferation and metastasis in colorectal carcinoma[J]. Cell Death Dis, 2021, 12(5): 443. doi: 10.1038/s41419-021-03714-8
[8]	董江萌, 王勇, 齐义新, 等. circRNA_001569通过miR-145/HBXIP轴影响乳腺癌细胞的恶性生物学行为[J]. 中国肿瘤生物治疗杂志, 2020, 27(11): 1220-1228. Dong JM, Wang Y, Qi YX, et al. circRNA_001569 affects the malignant biological behavior of breast cancer cells through the miR-145/HBXIP axis[J]. Zhongguo Zhong Liu Sheng Wu Zhi Liao Za Zhi, 2020, 27(11): 1220-1228.
[9]	Tian J, Xi X, Wang J, et al. CircRNA hsa_circ_0004585 as a potential biomarker for colorectal cancer[J]. Cancer Manag Res, 2019, 11: 5413-5423.
[10]	陈坤, 裘炯良, 张扬. 大肠癌危险因素的Meta分析[J]. 浙江大学学报(医学版), 2002, 31(4): 254-258. https://www.cnki.com.cn/Article/CJFDTOTAL-ZJYB200204007.htm Chen K, Qiu JL, Zhang Y. Meta-analysis study on risk factors of colorectal cancer[J]. Zhejiang Da Xue Xue Bao(Yi Xue Ban), 2002, 31(4): 254-258. https://www.cnki.com.cn/Article/CJFDTOTAL-ZJYB200204007.htm
[11]	Li XN, Wang ZJ, Ye CX, et al. Circular RNA circVAPA is up-regulated and exerts oncogenic properties by sponging miR-101 in colorectal cancer[J]. Biomed Pharmacother, 2019, 112: 108611.
[12]	Li XN, Wang ZJ, Ye CX, et al. RNA sequencing reveals the expression profiles of circRNA and indicates that circDDX17 acts as a tumor suppressor in colorectal cancer[J]. J Exp Clin Cancer Res, 2018, 37(1): 325.
[13]	李沛, 李颖, 刘宇. circRNA CDR1as靶向miR-87 6-5p调控肝癌细胞的增殖、迁移和侵袭[J]. 中国老年学杂志, 2021, 41(17): 3782-3786. Li P, Li Y, Liu Y. circRNA CDR1as targets miR-876-5p to regulate the proliferation, migration and invasion of liver cancer cells[J]. Zhongguo Lao Nian Xue Za Zhi, 41(17): 3782-3786.
[14]	何京, 张易, 杜明楠, 等. 血浆外泌体中Hsa_circ_0004071作为结直肠癌新诊断标志物的研究[J]. 徐州医科大学学报, 2020, 40(10): 715-719. He J, Zhang Y, Du MN, et al. Hsa_circ_0004071 in plasma exosomes as a new diagnostic marker for colorectal cancer[J]. Xuzhou Yi Ke Da Xue Xue Bao, 2020, 40(10): 715-719.
[15]	郭新园, 王蕊, 衣峻萱, 等. 环状RNA作为肿瘤标志物及其在肿瘤放疗中应用的研究进展[J]. 国际放射医学核医学杂志, 2020, 44(6): 374-380. Guo XY, Wang R, Yi JX, et al. Progress in the research on circRNAs as tumor markers and their applications in radiotherapy[J]. Guo Ji Fang She Yi Xue He Yi Xue Za Zhi, 2020, 44(6): 374-380.
[16]	向婧, 黄瑞雪. circRNA调控放疗抵抗相关通路的作用机制研究进展[J]. 中华放射医学与防护杂志, 2021, 41(4): 304-308. Xiang J, Huang RX. Research progress on the mechanism of circRNA regulating radiotherapy resistance-related pathways[J]. Zhonghua Fang She Yi Xue Yu Fang Hu Za Zhi, 2021, 41(4): 304-308.
[17]	Sherr CJ, Beach D, Shapiro GI. Targeting CDK4 and CDK6: From Discovery to Therapy[J]. Cancer Discov, 2016, 6(4): 353-367.
[18]	Pauklin S, Madrigal P, Bertero A, et al. Initiation of stem cell differentiation involves cell cycle-dependent regulation of developmental genes by Cyclin D[J]. Genes Dev, 2016, 30(4): 421-433.
[19]	Killinger M, Veselá B, Procházková M, et al. A single-cell analytical approach to quantify activated caspase-3/7 during osteoblast proliferation, differentiation, and apoptosis[J]. Anal Bioanal Chem, 2021, 413(20): 5085-5093.
[20]	García-Argüello SF, Lopez-Lorenzo B, Cornelissen B, et al. Development of [18F]ICMT-11 for Imaging Caspase-3/7 Activity during Therapy-Induced Apoptosis[J]. Cancers, 2020, 12(8): 2191.

[1]	DAI Nan, ZHAO Xiaolong, DAI Xiaoyan, LI Mengxia. Effect of Exosomal APE1 on Sensitivity of NSCLC A549 Cells to Cisplatin[J]. Cancer Research on Prevention and Treatment, 2020, 47(7): 492-497. DOI: 10.3971/j.issn.1000-8578.2020.19.1609
[2]	HUANG Zejian, FANG Chang, YU Baodan, CHENG Qing, LYU Ping. 3E10 Targeting CD24 Enhances Chemotherapy Sensitivity of Hepatocellular Carcinoma HuH-7 Cells[J]. Cancer Research on Prevention and Treatment, 2018, 45(8): 540-544. DOI: 10.3971/j.issn.1000-8578.2018.17.1484
[3]	CAI Rui, CHEN Qiuqiu, JIANG Wei. 5-azacytidine Increases Radiation Sensitivity of Nasopharyngeal Carcinoma Cell Line C666-1[J]. Cancer Research on Prevention and Treatment, 2017, 44(2): 94-97. DOI: 10.3971/j.issn.1000-8578.2017.02.003
[4]	ZHANG Juping, SHI Yehui, JIA Yongsheng, ZHOU Liyan, TONG Zhongsheng. GDF11 is Involved in Human Hepatic Carcinoma Cells SMMC-7721 Proliferation and Sensitivity to DDP[J]. Cancer Research on Prevention and Treatment, 2016, 43(6): 459-462. DOI: 10.3971/j.issn.1000-8578.2016.06.005
[5]	LI Wei, PENG Junqin, LI Jiansheng, TANG Rijie. MR Apparent Diffusion Coefficient Predicts Sensitivity of Nasopharyngeal Carcinoma to Radiotherapy and Related Factors[J]. Cancer Research on Prevention and Treatment, 2015, 42(12): 1221-1226. DOI: 10.3971/j.issn.1000-8578.2015.12.011
[6]	HU Lili, YIN Yanjun, ZHONG Wenjuan, QIU Feng. miR-200c Enhances Sensitivity of Lung Cancer Cell A549 to Paclitaxel and Gefitinib and Related Mechanism[J]. Cancer Research on Prevention and Treatment, 2015, 42(08): 760-764. DOI: 10.3971/j.issn.1000-8578.2015.08.003
[7]	CAO Xinmei, ZHANG Daiquan, XIA Jiyi, WANG Xu, GAO Yan, XIONG Wei. Effects of HER2 shRNA on Chemotherapy Sensitivity of Mouse Lewis Cells[J]. Cancer Research on Prevention and Treatment, 2014, 41(05): 366-368. DOI: 10.3971/j.issn.1000-8578.2014.05.004
[8]	Yang Qingshan, Liu Yuanyuan, Jiang Lipeng. Effect of Expression Vector of Human BAG-1 Gene on Radio-sensitivity of Lung Adenocarcinoma Cells[J]. Cancer Research on Prevention and Treatment, 2012, 39(02): 127-129. DOI: 10.3971/j.issn.1000-8578.2012.02.002
[9]	FANG Chuan, TAN Yan-li, WANG Jia-liang, SHI Yan-fang, SHAN Xiao-song, LI Wei. Primary Culture and Drug Sensitivity of Human Glioma Cells[J]. Cancer Research on Prevention and Treatment, 2010, 37(12): 1380-1382. DOI: 10.3971/j.issn.1000-8578.2010.12.012
[10]	ZHANG Wei, GU Min. Geldanamycin Sensitizes Human Breast Cancer Cells to Adriamycin both in vitro and in vivo[J]. Cancer Research on Prevention and Treatment, 2010, 37(10): 1109-1112. DOI: 10.3971/j.issn.1000-8578.2010.10.004

Cited By

Cited by

Periodical cited type(9)

1.	成俊，徐雪峰，李伟. D-CBCT在肺癌容积旋转调强计划精准治疗中的临床应用. 中国CT和MRI杂志. 2024(04): 35-37 .
2.	蒋浩，曹新超，吴桐，郭志斌. 低剂量CT联合外周血循环肿瘤细胞在肺癌早期诊断中的价值研究. 中国CT和MRI杂志. 2024(06): 57-60 .
3.	王晶，翟成凯. 肿瘤自身抗体及CT人工智能在NSCLC早期诊断中的应用研究进展. 解放军医学杂志. 2024(07): 848-854 .
4.	李雪娇. 多排螺旋CT低剂量胸部扫描在肺癌早期筛查中的应用价值分析. 医药前沿. 2024(15): 79-81 .
5.	易梅玲，易芳玲. 双层探测器光谱CT智能剂量调控技术在低剂量胸部体检中的应用价值. 中国医疗器械信息. 2024(22): 8-10+81 .
6.	杏子，彭源. 低剂量CT在肺癌筛查中的价值. 中国防痨杂志. 2024(S2): 234-236 .
7.	陈蔚，蒋伟. 低剂量螺旋CT扫描用于肺癌筛查及鉴别的效果评估. 深圳中西医结合杂志. 2023(01): 71-73 .
8.	慕珂珂，肖凌云，董炎红. 早期肺癌患者血清和胸腔积液中CYFRA211、NSE及SCC水平变化及意义. 中国实用医刊. 2023(09): 34-37 .
9.	陈彪. 低剂量螺旋CT联合血清肿瘤标志物在肺癌诊断中的临床应用价值. 影像研究与医学应用. 2023(22): 24-26 .

Other cited types(1)

Figures(6)

Get Citation

PDF

XML

Article views (1180) PDF downloads (233) Cited by(10)

Turn off MathJax

Article Contents

Abstract

References

Expression of Circular RNA hsa_circ_0018574 in Colorectal Cancer Tissues and Its Effect on Proliferation of Colorectal Cancer Cells