Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1

Objective  To explore the effect and mechanism of miR-581 on the autophagy of ovarian cancer SKOV3 cells.

Methods  miR-581 mimics and miR-581 NC were transfected into SKOV3 cells, and the transfection efficiency was detected by qRT-PCR. After successful transfection, Western blot was used to detect autophagy-related proteins expression in SKOV3 cells. TargetScanHuman database predicted miR-581 target genes, and Western blot verified the role of miR-581 and target genes.

Results  Overexpression of miR-581 significantly inhibited the expression of autophagy-related proteins LC3 Ⅱ and Beclin1 (P < 0.01); miR-581 negatively regulated FOXO1 expression (P < 0.01) and FOXO1 promoted autophagy of SKOV3 cells (P < 0.01).

Conclusion  miR-581 affects the autophagy of ovarian cancer SKOV3 cells by regulating the expression of FOXO1.