Cordycepin Attenuates Immune Function Injury in A Rat Model of Lung Cancer After Radiotherapy by Inhibiting JAK2/STAT3 Pathway

LIU Jianbo; LI Baiyu; KE Shanbao; ZHANG Wei

doi:10.3971/j.issn.1000-8578.2021.21.0094

Cancer Research on Prevention and Treatment > 2021 > 48(11): 994-998. > DOI: 10.3971/j.issn.1000-8578.2021.21.0094

LIU Jianbo, LI Baiyu, KE Shanbao, ZHANG Wei. Cordycepin Attenuates Immune Function Injury in A Rat Model of Lung Cancer After Radiotherapy by Inhibiting JAK2/STAT3 Pathway[J]. Cancer Research on Prevention and Treatment, 2021, 48(11): 994-998. DOI: 10.3971/j.issn.1000-8578.2021.21.0094

Citation:

PDF (3790 KB)

Cordycepin Attenuates Immune Function Injury in A Rat Model of Lung Cancer After Radiotherapy by Inhibiting JAK2/STAT3 Pathway

Cancer Center of Henan Provincial People's Hospital, Zhengzhou 450000, China

Funding:

Henan Medical Science and Technology Research Project LHGJ20190607

More Information

Received Date: January 24, 2021
Revised Date: July 25, 2021
Available Online: January 12, 2024

Graphical Abstract

Abstract

Abstract

Objective
To investigate the inhibitory effect of cordycepin on immune function injury in lung cancer rats after radiation therapy through JAK2/STAT3 signaling pathway.
Methods
Fifty rats were used to establish tumor-bearing model and other 10 rats were taken as normal group. After successful modeling, the rats were randomly divided into model group, radiotherapy group, cordycepin group, agonist group and agonist+cordycepin group (10 rats in each group). We compared tumor weight, tumor volume, tumor inhibition rate, IL-6, TNF-α, spleen index and thymus index, the number of T lymphocyte subsets, JAK2, p-JAK2, STAT3 and p-STAT3 protein expression levels among all groups.
Results
Compared with normal group, IL-6, TNF-α, CD8+, p-JAK2 and p-STAT3 in model group were increased, while spleen index, thymus index, CD4+ and CD4+/CD8+ were decreased (P < 0.05). Compared with model group, tumor weight, tumor volume, spleen index, thymus index, CD4+ and CD4+/CD8+ were decreased in radiotherapy group, while IL-6, TNF-α, CD8+, p-JAK2 and p-STAT3 were increased (P < 0.05). Compared with radiotherapy group, tumor weight, tumor volume, IL-6, TNF-α, CD8+, p-JAK2 and p-STAT3 were decreased in cordycepin group, while tumor inhibition rate, spleen index thymus index, CD4+ and CD4+/CD8+ were increased; tumor weight, tumor volume, IL-6, TNF-α, CD8+, p-JAK2 and p-STAT3 protein expression were increased in agonist group, while tumor inhibition rate, spleen index, thymus index, CD4+ and CD4+/CD8+ were decreased (P < 0.05). Compared with agonist+cordycepin group, tumor weight, tumor volume, IL-6, TNF-α, CD8+, p-JAK2 and p-STAT3 were decreased in cordycepin group, while tumor inhibition rate, spleen index, thymus index, CD4+ and CD4+/CD8+ were increased; tumor weight, tumor volume, IL-6, TNF-α, CD8+, p-JAK2 and p-STAT3 were increased in agonist group, while tumor inhibition rate, spleen index, thymus index, CD4+ and CD4+/CD8+ were decreased (P < 0.05).
Conclusion
Cordycepin can effectively inhibit the immune function injury in lung cancer rats after radiation therapy, and may play a regulatory role by inhibiting the JAK2/STAT3 signal pathway.
- Cordycepin,
- Radiation therapy,
- Immune function,
- JAK2/STAT3

FullText(HTML)

Competing interests: The authors declare that they have no competing interests.

References (19)

References

[1]	宋春洋, 祝淑钗, 沈文斌, 等. 临床Ⅲ期食管癌患者放疗前后免疫功能及外周血炎症指标对预后的影响分析[J]. 中华放射医学与防护杂志, 2020, 40(3): 189-195. doi: 10.3760/cma.j.issn.0254-5098.2020.03.006 Song CY, Zhu SC, Shen WB, et al. Analysis of the effects of immunity index and blood inflammatory markers pre- and post-radiotherapy on prognosis of clinical stage Ⅲ esophageal cancer patients[J]. Zhonghua Fang She Yi Xue Yu Fang Hu Za Zhi, 2020, 40(3): 189-195. doi: 10.3760/cma.j.issn.0254-5098.2020.03.006
[2]	王多, 鲍荣, 王芳, 等. 虫草素抗肿瘤作用分子机制研究进展[J]. 中国药理学与毒理学杂志, 2015, 29(4): 643-650. doi: 10.3867/j.issn.1000-3002.2015.04.018 Wang D, Bao R, Wang F, et al. progress in molecular mechanisms of anticancer action of corycepin[J]. Zhongguo Yao Li Xue Yu Du Li Xue Za Zhi, 2015, 29(4): 643-650. doi: 10.3867/j.issn.1000-3002.2015.04.018
[3]	Zhang Y, Zhang XX, Yuan RY, et al. Cordycepin induces apoptosis in human pancreatic cancer cells via the mitochondrial-mediated intrinsic pathway and suppresses tumor growth in vivo[J]. Onco Targets Ther, 2018, 11: 4479-4490. doi: 10.2147/OTT.S164670
[4]	Wang C, Mao ZP, Wang L, et al. Cordycepin inhibits cell growth and induces apoptosis in human cholangiocarcinoma[J]. Neoplasma, 2017, 64(6): 834-839. doi: 10.4149/neo_2017_604
[5]	Lin YT, Liang SM, Wu YJ, et al. Cordycepin suppresses endothelial cell proliferation, migration, angiogenesis, and tumor growth by regulating focal adhesion kinase and p53[J]. Cancers(Basel), 2019, 11(2): 168. http://www.onacademic.com/detail/journal_1000041694748599_3052.html
[6]	韩丹, 崔琳琳, 王莹, 等. 虫草素药理作用研究进展[J]. 食品工业科技, 2019, 40(9): 335-338, 345. https://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ201909057.htm Han D, Cui LL, Wang Y, et al. Research Progress On Pharmacological Effects Of Cordycepin[J]. Shi Pin Gong Ye Ke Ji, 2019, 40(9): 335-338, 345. https://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ201909057.htm
[7]	Mao Y, Yang D, He J, et al. Epidemiology of lung cancer[J]. Surg Oncol Clin N Am, 2016, 25(3): 439-445. doi: 10.1016/j.soc.2016.02.001
[8]	Michaelidesová A, Konířová J, Bartůněk P, et al. Effects of radiation therapy on neural stem cells[J]. Genes(Basel), 2019, 10(9): 640. http://www.ncbi.nlm.nih.gov/pubmed/31450566
[9]	Kirova Y, Tallet A, Aznar MC, et al. Radio-induced cardiotoxicity: from physiopathology and risk factors to adaptation of radiotherapy treatment planning and recommended cardiac follow-up[J]. Cancer Radiother, 2020, 24(6-7): 576-585. doi: 10.1016/j.canrad.2020.07.001
[10]	Weichselbaum RR, Liang H, Deng L, et al. Radiotherapy and immunotherapy: a beneficial liaison?[J]. Nat Rev Clin Oncol, 2017, 14(6): 365-379. doi: 10.1038/nrclinonc.2016.211
[11]	Woolley VC, Teakle GR, Prince G, et al. Cordycepin, a metabolite of Cordyceps militaris, reduces immune-related gene expression in insects[J]. J Invertebr Pathol, 2020, 177: 107480. doi: 10.1016/j.jip.2020.107480
[12]	Lei J, Wei Y, Song P, et al. Cordycepin inhibits LPS-induced acute lung injury by inhibiting inflammation and oxidative stress[J]. Eur J Pharmacol, 2018, 818: 110-114. doi: 10.1016/j.ejphar.2017.10.029
[13]	Tan L, Song X, Ren Y, et al. Anti-inflammatory effects of cordycepin: A review[J]. Phytother Res, 2020. Online ahead of print.
[14]	安青, 何立巍, 吴红雁, 等. 虫草素通过调节CD4+T淋巴细胞PD-1受体促进肿瘤免疫及机制研究[J]. 南京中医药大学学报, 2018, 34(5): 495-498. https://www.cnki.com.cn/Article/CJFDTOTAL-NJZY201805015.htm An Q, He LW, Wu HY, et al. Mechanism of cordycepin promotes tumor immunity by regulating PD-1 receptor of CD4+T lymphocytes[J]. Nanjing Zhong Yi Yao Da Xue Xue Bao, 2018, 34(5): 495-498. https://www.cnki.com.cn/Article/CJFDTOTAL-NJZY201805015.htm
[15]	Zundler S, Neurath MF. Integrating immunologic signaling networks: the JAK/STAT pathway in colitis and colitis-associated cancer[J]. Vaccines(Basel), 2016, 4(1): 5. http://www.onacademic.com/detail/journal_1000040540984510_5b25.html
[16]	Yu T, Li Z, Xu L, et al. Anti-inflammation effect of Qingchang suppository in ulcerative colitis through JAK2/STAT3 signaling pathway in vitro and in vivo[J]. J Ethnopharmacol, 2021, 266: 113442. doi: 10.1016/j.jep.2020.113442
[17]	Qing Y, Stark GR. Alternative activation of STAT1 and STAT3 in response to interferon-gamma[J]. J Biol Chem, 2004, 279(40): 41679-41685. doi: 10.1074/jbc.M406413200
[18]	Lei RE, Shi C, Zhang PL, et al. IL-9 promotes proliferation and metastasis of hepatocellular cancer cells by activating JAK2/STAT3 pathway[J]. Int J Clin Exp Pathol, 2017, 10(7): 7940-7946. http://www.researchgate.net/publication/326058664_IL-9_promotes_proliferation_and_metastasis_of_hepatocellular_cancer_cells_by_activating_JAK2STAT3_pathway
[19]	Chen J, Zhang W, Xu Q, et al. Ang-(1-7) protects HUVECs from high glucose-induced injury and inflammation via inhibition of the JAK2/STAT3 pathway[J]. Int J Mol Med, 2018, 41(5): 2865-2878. http://www.spandidos-publications.com/ijmm/41/5/2865/download

Cited By

Cited by

Periodical cited type(13)

1.	吴杨，隋雨桐，李斌鹏，韩路拓，姜家康. 激酶/转录因子信号通路调控肺癌机制及中医药干预的研究进展. 世界中医药. 2025(01): 142-147+154 .
2.	曹家瑞，冯博，马纯政，陈伟霞，喻江凡，曹莎莎，张振予，欧阳文慧. 中医药调控JAK/STAT信号通路干预肺癌的机制研究进展. 中国实验方剂学杂志. 2025(09): 265-276 .
3.	梁帅，尹怡，刘湘花，汪保英，骆文龙，龙云凯，任振杰，王祥麒. 升降理肺消瘤汤对Lewis肺癌小鼠免疫炎性反应和JAK2/STAT3信号通路的影响. 辽宁中医药大学学报. 2024(04): 27-32 .
4.	张彩蝶，靳艳，张德德. 润肺益肾饮对肺癌荷瘤大鼠的抑瘤作用和肿瘤免疫微环境的影响. 天津医药. 2024(04): 362-366 .
5.	孙喜，王召路，贾谨睿，王梦洋，孙润卓，王鹏，史新娥. 虫草素及其在生猪养殖中的应用. 畜牧兽医杂志. 2024(04): 1-7 .
6.	兰春燕，杨小兰，贺雪峰，赵丹，杨海燕. 甘草苷对胃癌荷瘤小鼠免疫功能的调节作用及机制研究. 中国药房. 2024(15): 1862-1867 .
7.	张景淇，郭静，陈娅欣，蒲玥衡，向俊杰. 中药调控肺癌相关信号通路研究进展. 中国实验方剂学杂志. 2024(19): 233-244 .
8.	张孟恩，韩睿，徐超，庞训胜，王世琴. 地顶孢霉培养物在反刍动物生产中的应用研究进展. 中国畜牧杂志. 2024(12): 70-74 .
9.	高铭，丁美灵，雷紫琴，胡靖文，栾飞，曾南. 荆防败毒散及其中成药制剂研究进展. 中药药理与临床. 2023(05): 112-118 .
10.	朱亚兰，吕世文，曾晨欣，徐媛青. 苍术素对非小细胞肺癌细胞上皮间质转化的影响及机制研究. 浙江医学. 2023(10): 1013-1018 .
11.	陈才伟，陈家亮，李华娟，方芳，文方玲. 虫草素调节MAPK/AP-1信号通路对慢性阻塞性肺疾病大鼠肺组织损伤的影响. 临床肺科杂志. 2023(11): 1656-1661 .
12.	李翔子，王西双，范建伟，杨田野，王丽娟，孙颖，姚景春. 荆防合剂通过抑制JAK2-STAT3信号通路调节荨麻疹小鼠脾脏T淋巴细胞亚群的平衡. 中国中药杂志. 2022(20): 5473-5480 .
13.	沈栩岚，黄凌霞. 虫草素的抗癌机理. 蚕桑通报. 2022(02): 33-34 .

Other cited types(4)

Figures(2) / Tables(5)

Get Citation

PDF

XML

Article views (1598) PDF downloads (302) Cited by(17)

Turn off MathJax

Article Contents

Abstract

References

Cordycepin Attenuates Immune Function Injury in A Rat Model of Lung Cancer After Radiotherapy by Inhibiting JAK2/STAT3 Pathway