Low Glucose and Mannose Synergistically Inhibit Growth of Pancreatic Tumor

Objective To investigate the sensitivity of pancreatic cancer cells to mannose under various glucose concentrations and the mechanisms.

Methods CCK-8 cell viability and proliferation kit was used to detect the proliferation of pancreatic cancer cells under different concentrations of glucose and mannose. Trypan blue staining was used to calculate the survival rate of mannose-cultured cells. Western blot was used to observe the expression of PMI enzyme. A subcutaneous xenograft tumor model was established to test the efficacy of ketogenic diet combined with mannose.

Results With the decrease of glucose concentration, the concentration of mannose required for the significant inhibition of pancreatic cancer cell proliferation was also significantly reduced; mannose significantly induced the expression of PMI enzymes in high glucose culture, and the induction effect was weakened in low glucose. The ketogenic diet had an effect on lowering blood sugar and raising blood ketones, and the inhibitory effect on the xenograft combined with mannose was significant.

Conclusion Low glucose concentration enhances the tumor inhibitory effect of mannose, and the combination of ketogenic diet and mannose could improve the antitumor effect.