| Citation: |
HOU Xia, ZHOU Yongan, TIAN Shuxiong, LI Chao, LI Min, REN Ruirui, HAN Yaxin, CHENG Jianping, LI Xingxing, LI Zhe, BAI Yuan. Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2019, 46(12): 1062-1067. DOI: 10.3971/j.issn.1000-8578.2019.19.0346
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To investigate the expression and function of lncRNA XIST in MCF-7 breast cancer cells.
The expression of XIST in MCF 10A human normal breast epithelial cells and MCF-7 human breast cancer cells were detected by qRT-PCR. Transient transfection technique was used to overexpress XIST in MCF-7 cells. MTT assay and Transwell assay were used to detect the changes of proliferation, migration and invasion of MCF-7 cells after XIST overexpression in MCF-7 cells. Bioinformatics and qRT-PCR were used to predict and detect the changes of miRNA expression which have potential binding sites of XIST. qRT-PCR was used to detect the expression of XIST in MCF-7 cells which was transfected instantaneously by miR-130b-3p inhibitor.
XIST expression was decreased in MCF-7 cells, while miR-130b-3p expression was increased, compared with those in MCF-10A cells (both P < 0.001). Overexpression of XIST significantly inhibited the proliferation of MCF-7 cells (P < 0.001), promoted cell migration and invasion. And the expression level of miR-130b-3p in MCF-7 cells was reduced significantly after overexpression of XIST (P < 0.01), while the expression level of XIST in MCF-7 cells was increased significantly after down-expression of miR-130b-3p (P < 0.001).
The expression levels of XIST and miR-130b-3p in breast cancer MCF-7 cells are negatively correlated, and the overexpression of XIST could significantly inhibit the proliferation, and promote the migration and invasion of MCF-7 cells.
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