Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells

Objective To detect the expression of FBXL20 in gastric cancer cells, and determine its effect on the proliferation, migration and invasion of gastric cancer cells.

Methods The expression levels of FBXL20 in gastric cancer cell lines BGC-823, AGS, MKN-45 and normal gastric epithelial cell line GES-1 were determined by Western blot. FBXL20 expression in AGS cells was inhibited by targeting FBXL20 shRNA lentiviral vectors. CCK-8 assay and Transwell assay were used to evaluate the proliferation, migration and invasion of AGS cells. The expression of Wnt pathway-related proteins were determined by Western blot.

Results FBXL20 expression was significantly up-regulated in gastric cancer cell lines, compared with the normal gastric epithelial cell line (P < 0.05). Compared with the control group, AGS cells with FBXL20 knockdown had lower proliferation, migration and invasion abilities (P < 0.05). Inhibition of FBXL20 reduced ubiquitination degradation of GSK-3β, which increased the expression levels of GSK-3β, p-GSK-3β^Tyr216, and decreased the expression levels of β-catenin, p-β-catenin, Cyclin D1 and E-cadherin.

Conclusion FBXL20 may promote the proliferation, migration and invasion of gastric cancer cells by regulating Wnt pathway.