Influences of FGF3 and FGF10 on Migration and Invasion of Human Ovarian Cancer SKOV3 Cells and Relation Between These Influences and Wnt/β-catenin Signaling Pathway

Objective To explore the influences of FGF3 and FGF10 on migration and invasion of human ovarian cancer SKOV3 cells and relation between these influences and Wnt/β-catenin signaling pathway.

Methods Human ovarian cancer SKOV3 cells were cultured in vitro and treated by FGF3 and FGF10 synthetic small interfering RNA. Then we used real-time PCR and Western blot Method to detect the interference effect. And then we utilized Transwell assay to detect the changes of migration and invasion of human ovarian cancer SKOV3 cells after down-regulating FGF3 and FGF10 gene expression. Moreover, we exploited real-time PCR Method to detect changes of FGF3 and FGF10 gene after activating Wnt signaling pathway by LiCl.

Results Knockdown of FGF3和FGF10 could reduce the migration and invasion of SKOV3 cells, compared with NC group and untreated SKOV3 group(P < 0.05). The migration and invasion abilities of NC group did not change significantly, compared with untreated SKOV3 group(P > 0.05). FGF3 and FGF10 expression levels were increased significantly after activating the Wnt/β-catenin signaling pathway.

Conclusion FGF3 and FGF10 modulated by Wnt/β-catenin signaling pathway promote the migration and invasion abilities of human ovarian cancer SKOV3 cells.