Garcinone E Inhibits Proliferation, Migration and Invasion of Human Nasopharyngeal Carcinoma Cells

Objective  To explore the effect of Garcinone E, extracted from Garcinia oblongifolia Champ, on the proliferation, migration and invasion of nasopharyngeal carcinoma(NPC) HK1 and HONE1 cells and elucidate its possible mechanism.

Methods The effect of Garcinone E on the proliferation, migration and invasion of nasopharyngeal carcinoma HK1 and HONE1 cells lines was examined using MTS and Transwell assay, respectively. The expressions of EMT-related protein and mRNA and possible pathway were detected by Western blot and q-PCR.

Results MTS assay showed that Garcinone E significantly inhibited the viability of HK1 and HONE1 cell lines in a dose-dependent manner. Transwell assay showed that compared with the blank control group, 7.5μmol/L Garcinone E suppressed the migration and invasion of HK1 and HONE1 cell lines obviously. Western blot results indicated that Garcinone E decreased the expression of p-Vimentin, Snail and Slug and increased the expression of E-cadherin in a dose-dependent manner. q-PCR assay showed that Garcinone E increased the expression of E-cadherin mRNA in HK1 and HONE1 cell lines.

Conclusion Garcinone E could inhibit the proliferation, migration and invasion abilities of nasopharyngeal carcinoma cells, which may be related to regulating the process of EMT.