Effects of Aloin on Proliferation, Apoptosis and Invasion of Esophageal Cancer KESY70 Cell Line

Objective To explore the effect of aloin on the proliferation, apoptosis and invasion of esophageal cancer KESY70 cell line.

Methods Different concentrations of aloin were added into KESY70 cells which were randomly divided into five groups: untreated group (KESY70), control group (DMSO) and Aloin groups (10, 40, 80 μmol/L). CCK-8 assay was used to test the viability and proliferation of KESY70 cells. The apoptosis was detected by flow cytometry. Transwell assay was performed to analyze the invasion of KESY70 cells. The expression levels of PCNA, cleaved caspase-3 and MMP-9 were checked by Western blot.

Results Cell viabilities of 40, 80 and 120 μmol/L Aloin groups were remarkably decreased, compared with DMSO group(P < 0.01). The proliferation rates of KESY70 cells treated with 40 and 80 μmol/L aloin for three days were attenuated, compared with the DMSO group (P < 0.05). Compared with DMSO group, the apoptosis rates were increased (P < 0.05), the invasion abilities were inhibited (P < 0.05), the expression levels of PCNA and MMP-9 were reduced (P < 0.05), and the expressions of cleaved caspase-3 were increased (P < 0.05) in Aloin groups.

Conclusion Aloin may suppress the proliferation, invasion and induce the apoptosis of KESY70 cells.