Influence of BCYRN1 on Proliferation and Migration of Breast Cancer Cell Line MCF7 and Tumor-bearing Mice

Objective To investigate the effect of BCYRN1 on proliferation and migration of breast cancer cell line MCF7 and transplatation tumor in mice.

Methods The breast cancer cell line MCF7 was transfected with BCYRN1 siRNA or siRNA scramble. Cell proliferation folds were tested by CCK-8. Protein expression was measured by Western blot. Wound healing assay was used to detect cell migration. The expression of proteins in breast cancer tissues was checked by immunohistochemistry staining.

Results Compared with control group, the cell proliferation folds in BCYRN1 siRNA group was attenuated (P=0.02). Moreover, the expressions of antigen identified by monoclonal antibody (Ki-67) and proliferating cell nuclear antigen (PCNA) were decreased (P=0.03). BCYRN1 siRNA treatment reduced the migration of breast cancer cells and repressed the expression of vascular endothelial growth factor (VEGF) and matrix metalloprotein 9 (MMP-9) (P=0.04). In vivo, the tumor volume of BCYRN1 siRNA group was smaller than that of control group (P=0.03); furthermore, the expression of Ki-67 and MMP-9 were decreased by BCYRN1 siRNA.

Conclusion BCYRN1 siRNA could suppress the proliferation and migration of breast cancer cell line MCF7 and transplantation tumor in mice.