Effect of Rutin Combined with Oxaliplatin on Activation of FKN/SYK/p38 Pathway to Promote Apoptosis of Gastric Cancer Cells

Objective To investigate the effect of rutin combined with oxaliplatin on the proliferation and apoptosis of human gastric cancer SGC-7901 cells and related mechanism.

Methods We took human gastric cancer SGC-7901 cells in the logarithmic growing period as the research object. MTT, flow cytometry and Western blot were used to detect cell viability, cell cycle and related protein expression.

Results P38 inhibitor could promote SGC-7901 cells proliferation(P < 0.05). Either rutin or oxaliplatin could inhibit the proliferation of SGC-7901 cells, and the inhibitory effect of their combination was more significant. The expressions of FKN, SYK, p-p38, cleaved-caspase3, cleaved-caspase7, cleaved-caspase8 and cleaved-caspase9 protein in p38 inhibitor group were significantly lower than those in negative control group(P < 0.05); the above proteins expressions were increased in varying degrees after treated with rutin combined with oxaliplatin.

Conclusion Rutin and oxaliplatin could inhibit the proliferation and induce apoptosis of SGC-7901 cells. The mechanism may be related to the activation of FKN/SYK/p38 pathway.