miR-23a Targeting NID2 Regulates Invasion and Migration of Lung Cancer Cells Through Notch Pathway

Objective To investigate the effect of miR-23a targeting NID2 on the biological behavior of lung cancer cells through Notch pathway and related mechanism.

Methods Immunohistochemistry was used to detect the expression of NID2 in lung cancer tissues and adjacent normal lung tissues. The expressions of NID2 in different lung cancer cell lines were detected by Western blot. Luciferase reporter gene was used to detect the interaction between miR-23a and NID2. Transwell invasion assay and scratch assay were used to detect the effect of miR-23a expression on the invasion and migration abilities of lung cancer cells. The expression of hes1 and NICD were detected in H1650 lung cancer cell line by Western blot.

Results The expression of NID2 in lung cancer tissues was significantly higher than that in adjacent normal lung tissues. The expression level of NID2 in H1650 lung cancer cell line was the highest; miR-23a can regulate the expression of NID2. miR-23a can regulate the invasion and migration of lung cancer H1650 cells. The expression of hes1 and NICD were deceased in H1650 lung cancer cell line by the overexpression of miR-23a.

Conclusion miR-23a targeting NID2 can regulate the invasion and migration of lung cancer cells through Notch signaling pathway.