CHD1L Silencing Inhibits Proliferation and Invasion of Intrahepatic Cholangiocarci-noma Cell Lines

Objective To investigate the effect of CHD1L silencing by RNAi on the growth and invasion of intrahepatic cholangiocarcinoma(ICC) cells line.

Methods The protein and mRNA expressions of CHD1L in three types of ICC cell lines, RBE, HCCC-9810 and HUCCT1, were determined by Western blot and qRT-PCR. The overexpression of CHD1L cell line was selected and after transfection with CHD1L-RNAi, the protein expressions of CHD1L were measured by Western blot. CCK-8 assay and plate clone formation assay were used to define the cell proliferation rate. Cell migration and invasion was detected by cell scratch assay and Transwell migration and invasion assay.

Results The protein and mRNA expressions of CHD1L in RBE cell line were significantly higher than those in HCCC-9810, HUCCT1 cell lines (P=0.003, P=0.012 and P=0.008, P=0.015). After CHD1L-RNAi was transfected into RBE cell line, we found that the interference effect of CHD1L-RNAi1 was significantly better than those of CHD1L-RNAi2 and CHD1L-RNAi3 (P=0.003, P=0.008). CCK-8 results showed CHD1L-RNAi1 group could obviously inhibit the proliferation of RBE cells line (P < 0.01). Meantime, the results of plate clone formation assay, cell scratch and Transwell migration and invasion experiment showed the number of colony formation, the rate of migration, the cells number of migration and invasion of CHD1L-RNAi1 group were significantly lower than those in normal and negative control groups (P < 0.05).

Conclusion C H D1L silencing by CHD1L-RNAi could inhibit the proliferation, migration and invasion of RBE cells line.