Effects of siRNA Targeting YKL-40 Gene on Chemosensitivity of Endometrial Cancer HEC-1A Cells to Cisplatin

Objective To explore the effects of silencing YKL-40 gene by small interfering RNA (siRNA) on the chemosensitivity of endometrial cancer HEC-1A cells to cisplatin.

Methods We silenced the YKL-40 gene in HEC-1A cells using the siRNA technique and divided the HEC-1A cells into three groups as blank control group, mock-treatment group and experimental group, respectively. The mRNA expression of YKL-40 was measured using quantitative real time-PCR(qRT-PCR) assay after treated with cisplatin. The proliferation and apoptosis of HEC-1A cells were also determined using MTT assay and flow cytometry(FCM) respectively.

Results The mRNA expression of YKL-40 in the experimental group was significantly lower than those in blank control and mock-treatment groups(P=0.036, 0.005, respectively). The mRNA expression of YKL-40 in HEC-1A cells was up-regulated after treatment of HEC-1A cells with the same concentration of cisplatin. The proliferative ability of HEC-1A cells was decreased(P < 0.05) while the apoptosis was increased(P=0.000) significantly in the experimental group, compared with the other two groups.

Conclusion The chemosensitivity of HCE-1A cells to cisplatin could be elevated by silencing YKL-40 gene. YKL-40 gene in HCE-1A cells have the ability of anti-apoptosis.