| Citation: |
ZHANG Wei, ZHAO Xuehui, RUAN Lalin, LI Yuecheng, FU Xiaoling, WU Kai, CHEN Weigao. Expression of miRNA-130b in Osteosarcoma Tissues and Its Impact on Proliferation and Apoptosis of Osteosarcoma Cells[J]. Cancer Research on Prevention and Treatment, 2017, 44(5): 334-339. DOI: 10.3971/j.issn.1000-8578.2017.05.005
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To detect the expression of miRNA-130b in osteosarcoma tissues and investigate the effect of miRNA-130b on the proliferation, cell cycle and apoptosis of human osteosarcoma cell line U2.
Real-time PCR was used to detect the expression level of miRNA-130b in osteosarcoma tissues and corresponding paratumorous tissues collected from 48 patients.The expression level of miRNA-130b in osteosarcoma cell line U2 was measured by qRT-PCR after transfected with miRNA-130b inhibitor. The cell proliferation was determined by CCK8 assay.Flow cytometry was used to monitor cell cycle and apoptosis. Luciferase gene reporter assay was used to detect the regulation mechanism of miRNA-130b and RUNX3. The change of RUNX3 and miRNA-130b of U2 cells after transfection was detected.
miRNA-130b was highly expressed in osteosarcoma tissues, compared with the adjacent normal tissues. The miRNA-130b expression of U2 cells after transfection was significantly down-regulated. The proliferation activity of U2 cells was inhibited, the cell cycle was arrested in G0/G1 phase, and the cell apoptosis was increased after transfected with miRNA-130b inhibitor. We found that miRNA-130b reduced wide-type RUNX3 3′-UTR luciferase expression intensity. The relative expression of RUNX3 in miRNA-130b inhibitor transfection group was significantly higher than those in negative control group. The relative expression of miRNA-130b in RUNX3 siRNA transfection group had no significant difference with those in negative control group.
miRNA-130b is highly expressed in human osteosarcoma tissue. miRNA-130b may inhibit the proliferation and induce the apoptosis of osteosarcoma cell line U2 via inhibiting the RUNX3 expression in vitro.
| [1] |
Chui MH, Kandel RA, Wong M, et al.Histopathologic Features of Prognostic Significance in High-Grade Osteosarcoma[J].Arch Pathol Lab Med, 2016.[Epub ahead of print] https://www.researchgate.net/publication/306434754_Histopathologic_Features_of_Prognostic_Significance_in_High-Grade_Osteosarcoma
|
| [2] |
Ottaviani G, Jaffe N.The epidemiology of osteosarcoma[J].Cancer Treat Res, 2009, 152: 3-13. doi: 10.1007/978-1-4419-0284-9
|
| [3] |
Bueno MJ, Pérez de Castro I, Malumbres M.Control of cell proliferation pathways by microRNAs[J].Cell Cycle, 2008, 7(20): 3143-8. doi: 10.4161/cc.7.20.6833
|
| [4] |
Fan YH, Ye MH, Wu L, et al.Overexpression of miR-98 inhibits cell invasion in glioma cell lines via downregulation of IKKepsilon[J].Eur Rev Med Pharmacol Sci, 2015, 19(19): 3593-604. https://www.researchgate.net/publication/292378051_Overexpression_of_miR-98_inhibits_cell_invasion_in_glioma_cell_lines_via_downregulation_of_IKK_epsilon
|
| [5] |
Volk N, Shomron N.Versatility of MicroRNA biogenesis[J].PLoS One, 2011, 6(5): e19391. doi: 10.1371/journal.pone.0019391
|
| [6] |
Gong XC, Xu YQ, Jiang Y, et al.Onco-microRNA miR-130b promoting cell growth in children APL by targeting PTEN[J].Asian Pac J Trop Med, 2016, 9(3): 265-8. doi: 10.1016/j.apjtm.2016.01.024
|
| [7] |
Chen C, Hu Y, Li L.NRP1 is targeted by miR-130a and miR-130b, and is associated with multidrug resistance in epithelial ovarian cancer based on integrated gene network analysis[J].Mol Med Rep, 2016, 13(1): 188-96. https://www.researchgate.net/publication/256290853_MicroRNA_and_transcription_factor_mediated_regulatory_network_for_ovarian_cancer_Regulatory_network_of_ovarian_cancer
|
| [8] |
Siragam V, Rutnam ZJ, Yang W, et al.MicroRNA miR-98 inhibits tumor angiogenesis and invasion by targeting activin receptor-like kinase-4 and matrix metalloproteinase-11[J].Oncotarget, 2012, 3(11): 1370-85. doi: 10.18632/oncotarget
|
| [9] |
Dong J, Liu Y, Liao W, et al.miRNA-223 is a potential diagnostic and prognostic marker for osteosarcoma[J].J Bone Oncol, 2016, 5(2): 74-9. doi: 10.1016/j.jbo.2016.05.001
|
| [10] |
Chen J, Zhou J, Chen X, et al.miRNA-449a is downregulated in osteosarcoma and promotes cell apoptosis by targeting BCL2[J].Tumour Biol, 2015, 36(10): 8221-9. doi: 10.1007/s13277-015-3568-y
|
| [11] |
Chen D, Cabay RJ, Jin Y, et al.MicroRNA Deregulations in Head and Neck Squamous Cell Carcinomas[J].J Oral Maxillofac Res, 2013, 4(1): e2. http://www.oalib.com/paper/2844610
|
| [12] |
张瑞, 徐修鹏, 陈正新, 等.miR-130b在胶质瘤中表达及其对胶质瘤增殖与侵袭的影响[J].江苏医药, 2014, 40(16): 1864-7. http://www.cnki.com.cn/Article/CJFDTOTAL-YIYA201416002.htm
Zhang R, XU XP, Chen ZX, et al.Exepression of miR-130b in glioblastoma and its impact on proliferation and invasion of human glioma cells[J].Jiangsu Yi Yao, 2014, 40(16): 1864-7. http://www.cnki.com.cn/Article/CJFDTOTAL-YIYA201416002.htm
|
| [13] |
郁婷婷, 李硕, 傅敏根, 等.miR-130b在食管鳞癌中的表达及对食管鳞癌细胞增殖和迁移的影响[J].世界华人消化杂志, 2013, 21(18): 1685-92. http://www.cnki.com.cn/Article/CJFDTOTAL-XXHB201318003.htm
Yu TT, Li S, Fu MG, et al.Expression of miR-130b in esophageal squamous cell carcinoma and effect of miR-130b transfection on cell proliferation and migration in an esophageal squamous cell carcinoma cell line[J].Shi Jie Hua Ren Xiao Hua Za Zhi, 2013, 21(18): 1685-92. http://www.cnki.com.cn/Article/CJFDTOTAL-XXHB201318003.htm
|
| [14] |
Feigh M, Hjuler ST, Andreassen KV, et al.Oral salmon calcitonin enhances insulin action and glucose metabolism in diet-induced obese streptozotocin-diabetic rats[J].Eur J Pharmacol, 2014, 737: 91-6. doi: 10.1016/j.ejphar.2014.05.016
|
| [15] |
吴同申, 彭圣智, 孟彦.流式细胞术检测骨肉瘤中RUNX3和β-catenin的表达及意义[J].中国现代医生, 2012, 50(32): 4-5. http://www.cnki.com.cn/Article/CJFDTOTAL-ZDYS201232004.htm
Wu TS, Peng SZ, Meng Y.Expression and significance of RUNX3 and β-catenin in osteosarcoma by FCM[J].Zhongguo Xian Dai Yi Sheng, 2012, 50(32): 4-5. http://www.cnki.com.cn/Article/CJFDTOTAL-ZDYS201232004.htm
|
| [16] |
刘融, 陈令斌, 张勇.骨肉瘤外周血Runx3基因启动子甲基化检测及临床意义[J].检验医学与临床, 2015, 7: 978-9. doi: 10.3969/j.issn.1672-9455.2015.07.044
Liu R, Chen LB, Zhang Y.Detection of Runx3 gene promoter methylation in peripheral blood of osteosarcoma and its clinical significance[J].Jian Yan Yi Xue Yu Lin Chuang, 2015, 7: 978-9. doi: 10.3969/j.issn.1672-9455.2015.07.044
|
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