Effect of Nuclear Franscription Factor Snail on Invasion and Migration of Intrahepatic Cholangiocarcinoma Cell Line HCCC9810

Objective To investigate the effect of nuclear transcription factor Snail on the invasion and migration of HCCC9810 cell line.

Methods The intrahepatic cholangiocarcinoma cell line HCCC9810 was selected as the experimental cell. Small interfering RNA (si-RNA) was used to target the silencing Snail. The invasion and migration abilities of intrahepatic cholangiocarcinoma cells were detected by Transwell and scratch test. The expression of E-cadherin and Vimentin which were epithelial mesenchymal transition (EMT) associated protein in cholangiocarcinoma cells treated with si-RNA were detected by immunoblotting.

Results The migration and invasion abilities of HCCC9810 cell were decreased after the targeted silencing of Snail (P=0.005; P=0.007). Cell scratch results showed that cell migration was also decreased (P=0.017). Immunoblotting results showed that E-cadherin expression was up-regulated (P=0.004) after silencing Snail, whereas Vimentin expression was down-regulated (P=0.001).

Conclusion Snail could induce epithelial mesenchymal transition in HCCC9810 cells, which can enhance invasion and migration abilities of HCCC9810 cells.