Effects of Ritonavir on Expression of LYN in Kaposi's Sarcoma-associated Herpesvirus-infected Cells

Objective To detect the effects of protein inhibitor ritonavir (RTV) on the expression of LYN in Kaposi' s sarcoma associated herpesvirus (KSHV) infected cells.

Methods Primary Human Umbilical Vein Endothelial Cells (HUVECs) were cultivated by enzyme infusion method. KSHV were extracted from BCBL-1 cells and infected HUVECs. PCR was used to amplify KS330₂₃₃ fragment to verify the KSHV infection. We used CCK8 method to detect the IC₅₀ of RTV on HUVECs and the proliferation ability of the cells. Western blot was used to detect LYN expression, p-PI3K and p-AKT levels.

Results RTV could inhibit the growth of KSHV-infected HUVECs in a concentration-dependent manner (P < 0.05). The IC₅₀ of RTV effect on cells was 25μmol/L. RTV could inhibit LYN, p-PI3K and p-AKT expression.

Conclusion RTV could inhibit the proliferation of KSHV-infected HUVECs through inhibiting LYN and PI3K/AKT signaling pathways.